![[KO Validated] TSC22D3 Rabbit pAb (A19911)](/upload/resize_cache/iblock/e43/340_340_140cd750bba9870f18aada2478b24840a/7o5fkba0a8lbppd2dsk6ycjbhor3pzax.jpg "[KO Validated] TSC22D3 Rabbit pAb (A19911)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] TSC22D3 Rabbit pAb (A19911)
- Описание
- Характеристики
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Overview
Product name [KO Validated] TSC22D3 Rabbit pAb Catalog No. A19911 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes the anti-inflammatory protein glucocorticoid (GC)-induced leucine zipper. Expression of this gene stimulated by glucocorticoids and interleukin 10 and it appears to play a key role in the anti-inflammatory and immunosuppressive effects of this steroid. This protein has also been shown to inhibit pro-inflammatory molecules including nuclear factor κB. Alternate splicing results in multiple transcript variants.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-79 of human TSC22D3 (NP_001015881.1). Sequence MDLVKNHLMYAVREEVEILKEQIRELVEKNSQLERENTLLKTLASPEQLEKFQSCLSPEEPAPESPQVPEAPGGSAV Gene ID Swiss Prot Synonyms DIP; GILZ; DSIPI; TSC-22R Calculated MW 15kDa Observed MW 16kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC IP Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
- IP 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Immunoprecipitation Positive samples 293T Cellular location Cytoplasm, Nucleus 
Western blot analysis of extracts from normal (control) and TSC22D3 knockout (KO) 293T cells, using TSC22D3 antibody (A19911) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s. -
Key applications Western blotting, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-79 of human TSC22D3 (NP_001015881.1). KO Validated Validated Isotype IgG