![[KO Validated] TIMM17A Rabbit pAb (A19917)](/upload/resize_cache/iblock/5e3/340_340_140cd750bba9870f18aada2478b24840a/yb3mrdaukoqs0m6zufkc9k9kbkavwzvf.jpg "[KO Validated] TIMM17A Rabbit pAb (A19917)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] TIMM17A Rabbit pAb (A19917)
- Описание
- Характеристики
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Overview
Product name [KO Validated] TIMM17A Rabbit pAb Catalog No. A19917 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Predicted to contribute to protein transmembrane transporter activity. Predicted to be involved in protein import into mitochondrial matrix. Located in mitochondrial inner membrane and nucleoplasm. Is integral component of mitochondrial inner membrane. Part of TIM23 mitochondrial import inner membrane translocase complex.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-171 of human TIMM17A (NP_006326.1). Sequence MEEYAREPCPWRIVDDCGGAFTMGTIGGGIFQAIKGFRNSPVGVNHRLRGSLTAIKTRAPQLGGSFAVWGGLFSMIDCSMVQVRGKEDPWNSITSGALTGAILAARNGPVAMVGSAAMGGILLALIEGAGILLTRFASAQFPNGPQFAEDPSQLPSTQLPSSPFGDYRQYQ Gene ID Swiss Prot Synonyms TIM17; TIM17A Calculated MW 18kDa Observed MW 16kDa Applications
Reactivity Human, Mouse Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples 293T Cellular location Mitochondrion inner membrane, Multi-pass membrane protein 
Western blot analysis of extracts from normal (control) and TIMM17A knockout (KO) 293T cells, using TIMM17A antibody (A19917) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-171 of human TIMM17A (NP_006326.1). KO Validated Validated Isotype IgG