![[KO Validated] TOMM34 Rabbit pAb (A19960)](/upload/resize_cache/iblock/cfd/340_340_140cd750bba9870f18aada2478b24840a/k1xroo89qxlu8dstkymunq9wa9p6h1ab.jpg "[KO Validated] TOMM34 Rabbit pAb (A19960)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] TOMM34 Rabbit pAb (A19960)
- Описание
- Характеристики
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Overview
Product name [KO Validated] TOMM34 Rabbit pAb Catalog No. A19960 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is involved in the import of precursor proteins into mitochondria. The encoded protein has a chaperone-like activity, binding the mature portion of unfolded proteins and aiding their import into mitochondria. This protein, which is found in the cytoplasm and sometimes associated with the outer mitochondrial membrane, has a weak ATPase activity and contains 6 TPR repeats.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human TOMM34 (NP_006800.2). Sequence MAPKFPDSVEELRAAGNESFRNGQYAEASALYGRALRVLQAQGSSDPEEESVLYSNRAACHLKDGNCRDCIKDCTSALALVPFSIKPLLRRASAYEALEKYPMAYVDYKTVLQIDDNVTSAVEGINRMTRALMDSLGPEWRLKLPSIPLVPVSAQKRWNSLPSENHKEMAKSKSKETTATKNRVPSAGDVEKARVLKEEGNELVKKGNHKKAIEKYSESLLCSNLESATYSNRALCYLVLKQYTEAVKDCTEALKLDGKNVKAFYRRAQAHKALKDYKSSFADISNLLQIEPRNGPAQKLRQEVKQNLH Gene ID Swiss Prot Synonyms TOM34; URCC3; HTOM34P Calculated MW 35kDa Observed MW 34kDa Applications
Reactivity Human, Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa Cellular location Cytoplasm, Cytoplasmic side, Mitochondrion outer membrane, Peripheral membrane protein 
Western blot analysis of extracts from normal (control) and TOMM34 knockout (KO) HeLa cells, using TOMM34 antibody (A19960) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human TOMM34 (NP_006800.2). KO Validated Validated Isotype IgG