![[KO Validated] PDXK Rabbit pAb (A21693)](/upload/resize_cache/iblock/a64/340_340_140cd750bba9870f18aada2478b24840a/kpllohh8klb25lvwke5y8no80n7mpfhp.jpg "[KO Validated] PDXK Rabbit pAb (A21693)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] PDXK Rabbit pAb (A21693)
- Описание
- Характеристики
-
Overview
Product name [KO Validated] PDXK Rabbit pAb Catalog No. A21693 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-312 of human PDXK (NP_003672.1). Sequence MEEECRVLSIQSHVIRGYVGNRAATFPLQVLGFEIDAVNSVQFSNHTGYAHWKGQVLNSDELQELYEGLRLNNMNKYDYVLTGYTRDKSFLAMVVDIVQELKQQNPRLVYVCDPVLGDKWDGEGSMYVPEDLLPVYKEKVVPLADIITPNQFEAELLSGRKIHSQEEALRVMDMLHSMGPDTVVITSSDLPSPQGSNYLIVLGSQRRRNPAGSVVMERIRMDIRKVDAVFVGTGDLFAAMLLAWTHKHPNNLKVACEKTVSTLHHVLQRTIQCAKAQAGEGVRPSPMQLELRMVQSKRDIEDPEIVVQATVL Gene ID Swiss Prot Synonyms PKH; PNK; HMSN6C; PRED79; C21orf97; HEL-S-1a; C21orf124 Calculated MW 35kDa Observed MW Refer to figures Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples Cellular location Cytoplasm 
Western blot analysis of extracts from normal (control) and PDXK knockout (KO) 293T cells, using PDXK antibody (A21693) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunofluorescence analysis of NIH-3T3 using PDXK antibody (A21693) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa using PDXK antibody (A21693) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa using PDXK antibody (A21693) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
-
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-312 of human PDXK (NP_003672.1). KO Validated Validated Isotype IgG