![[KO Validated] PTPN1 Rabbit pAb (A22243)](/upload/resize_cache/iblock/92c/340_340_140cd750bba9870f18aada2478b24840a/kbwwdcpus9f3hmxc30ad0qg5nb32v762.jpg "[KO Validated] PTPN1 Rabbit pAb (A22243)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] PTPN1 Rabbit pAb (A22243)
- Описание
- Характеристики
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Overview
Product name [KO Validated] PTPN1 Rabbit pAb Catalog No. A22243 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant protein of human PTPN1 Sequence Email for sequence Gene ID Swiss Prot Synonyms PTP1B Calculated MW 50kDa Observed MW 50kDa Applications
Reactivity Human, Rat Tested applications WB Recommended dilution - WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, C6 Cellular location cytoplasm, cytosol, early endosome, endoplasmic reticulum, endosome lumen, mitochondrial crista, mitochondrial matrix, sorting endosome 
Western blot analysis of HeLa, using PTPN1 antibody (A22243) at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of C6, using PTPN1 antibody (A22243) at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of C6, using PTPN1 antibody (A22243) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts from wild type(WT) and PTPN1 knockout (KO) HeLa(KO) cells, using PTPN1 antibody (A22243) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Крыса Immunogen Recombinant protein of human PTPN1 KO Validated Validated Isotype IgG