![[KO Validated] PMS2 Rabbit mAb (A22350)](/upload/resize_cache/iblock/418/340_340_140cd750bba9870f18aada2478b24840a/zg43yfp9rxqsxci8kmebpbl7350gt0dx.jpg "[KO Validated] PMS2 Rabbit mAb (A22350)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] PMS2 Rabbit mAb (A22350)
- Описание
- Характеристики
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Overview
Product name [KO Validated] PMS2 Rabbit mAb Catalog No. A22350 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2479 Background
The protein encoded by this gene is a key component of the mismatch repair system that functions to correct DNA mismatches and small insertions and deletions that can occur during DNA replication and homologous recombination. This protein forms heterodimers with the gene product of the mutL homolog 1 (MLH1) gene to form the MutL-alpha heterodimer. The MutL-alpha heterodimer possesses an endonucleolytic activity that is activated following recognition of mismatches and insertion/deletion loops by the MutS-alpha and MutS-beta heterodimers, and is necessary for removal of the mismatched DNA. There is a DQHA(X)2E(X)4E motif found at the C-terminus of the protein encoded by this gene that forms part of the active site of the nuclease. Mutations in this gene have been associated with hereditary nonpolyposis colorectal cancer (HNPCC; also known as Lynch syndrome) and Turcot syndrome.Immunogen information
Immunogen Recombinant protein of human PMS2. Sequence Email for sequence Gene ID Swiss Prot Synonyms MLH4; PMS-2; PMSL2; HNPCC4; LYNCH4; MMRCS4; PMS2CL Calculated MW 96kDa Observed MW Refer to figures Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples Jurkat cells, Hela cells Cellular location Nucleus 
Western blot analysis of extracts of Jurkat cells, using PMS2 antibody (A22350) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of extracts from wild type (WT) and PMS2 knockout (KO) HeLa cells, using PMS2 antibody (A22350) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded human appendix using PMS2 Rabbit mAb (A22350) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using PMS2 Rabbit mAb (A22350) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human pancreas using PMS2 Rabbit mAb (A22350) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human tonsil using PMS2 Rabbit mAb (A22350) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant protein of human PMS2. KO Validated Validated Isotype IgG