![[KO Validated] Smad3 Rabbit mAb (A23491)](/upload/resize_cache/iblock/742/340_340_140cd750bba9870f18aada2478b24840a/g421pjgbqcsqdsz9fn7bi1qng7c319py.jpg "[KO Validated] Smad3 Rabbit mAb (A23491)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] Smad3 Rabbit mAb (A23491)
- Описание
- Характеристики
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Overview
Product name [KO Validated] Smad3 Rabbit mAb Catalog No. A23491 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0490 Background
The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. This protein forms a complex with other SMAD proteins and binds DNA, functioning both as a transcription factor and tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3.Immunogen information
Immunogen Recombinant protein of human Smad3. Sequence Email for sequence Gene ID Swiss Prot Synonyms LDS3; mad3; LDS1C; MADH3; JV15-2; hMAD-3; hSMAD3; HSPC193; HsT17436 Calculated MW 48kDa Observed MW 52kDa Applications
Reactivity Human, Mouse Tested applications WB IHC-P IF/ICC IP Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
- IP 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation Positive samples 293T, A-431 Cellular location Cytoplasm, Nucleus 
Western blot analysis of extracts from wild type (WT) and Smad3 knockout (KO) 293T cells, using Smad3 antibody (A23491) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of extracts of A-431 cells, using Smad3 antibody (A23491) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded human liver using [KO Validated] Smad3 Rabbit mAb (A23491) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 300 μg extracts of A-431 cells using 3 μg Smad3 antibody (A23491). Western blot was performed from the immunoprecipitate using Smad3 antibody (A23491) at a dilution of 1:500.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant protein of human Smad3. KO Validated Validated Isotype IgG