![[KO Validated] SETDB1 Rabbit pAb (A6145)](/upload/resize_cache/iblock/3e3/340_340_140cd750bba9870f18aada2478b24840a/e01nhnpa6qm77m2llmjbaj1ricttzv4y.jpg "[KO Validated] SETDB1 Rabbit pAb (A6145)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] SETDB1 Rabbit pAb (A6145)
- Описание
- Характеристики
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Overview
Product name [KO Validated] SETDB1 Rabbit pAb Catalog No. A6145 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a histone methyltransferase which regulates histone methylation, gene silencing, and transcriptional repression. This gene has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. Alternatively spliced transcript variants of this gene have been described.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-397 of human SETDB1 (NP_001230420.1). Sequence MSSLPGCIGLDAATATVESEEIAELQQAVVEELGISMEELRHFIDEELEKMDCVQQRKKQLAELETWVIQKESEVAHVDQLFDDASRAVTNCESLVKDFYSKLGLQYRDSSSEDESSRPTEIIEIPDEDDDVLSIDSGDAGSRTPKDQKLREAMAALRKSAQDVQKFMDAVNKKSSSQDLHKGTLSQMSGELSKDGDLIVSMRILGKKRTKTWHKGTLIAIQTVGPGKKYKVKFDNKGKSLLSGNHIAYDYHPPADKLYVGSRVVAKYKDGNQVWLYAGIVAETPNVKNKLRFLIFFDDGYASYVTQSELYPICRPLKKTWEDIEDISCRDFIEEYVTAYPNRPMVLLKSGQLIKTEWEGTWWKSRVEEVDGSLVRILFLVLFFSTILEAEVGGGGT Gene ID Swiss Prot Synonyms ESET; KG1T; KMT1E; TDRD21; H3-K9-HMTase4 Calculated MW 143kDa Observed MW 180kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T, HeLa, Mouse testis Cellular location Chromosome, Nucleus Customer validation WB(Rattus norvegicus)
Western blot analysis of extracts from normal (control) and SETDB1 knockout (KO) 293T cells, using SETDB1 antibody (A6145) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using SETDB1 antibody (A6145) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Western blot analysis of 293F, using TOM20 antibody (A6145) at 1:900 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-397 of human SETDB1 (NP_001230420.1). KO Validated Validated Isotype IgG