![[KO Validated] SUZ12 Rabbit pAb (A7786)](/upload/resize_cache/iblock/66f/340_340_140cd750bba9870f18aada2478b24840a/dnlp51cj6xxohcpnj6a44yisctr0xd33.jpg "[KO Validated] SUZ12 Rabbit pAb (A7786)")
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Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] SUZ12 Rabbit pAb (A7786)
- Описание
- Характеристики
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Overview
Product name [KO Validated] SUZ12 Rabbit pAb Catalog No. A7786 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 470-739 of human SUZ12 (NP_056170.2). Sequence CHSRFIFNYVYHPKGARIDVSINECYDGSYAGNPQDIHRQPGFAFSRNGPVKRTPITHILVCRPKRTKASMSEFLESEDGEVEQQRTYSSGHNRLYFHSDTCLPLRPQEMEVDSEDEKDPEWLREKTITQIEEFSDVNEGEKEVMKLWNLHVMKHGFIADNQMNHACMLFVENYGQKIIKKNLCRNFMLHLVSMHDFNLISIMSIDKAVTKLREMQQKLEKGESASPANEEITEEQNGTANGFSEINSKEKALETDSVSGVSKQSKKQKL Gene ID Swiss Prot Synonyms CHET9; IMMAS; JJAZ1 Calculated MW 83kDa Observed MW 83kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC IP Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:100
- IP 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Immunoprecipitation Positive samples HeLa, BT-474, MCF-7, 293T Cellular location Nucleus Customer validation WB(Homo sapiens)
IP(Homo sapiens)
IP(Homo sapiens)
Western blot analysis of extracts of various cell lines, using SUZ12 Antibody (A7786) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of extracts from normal (control) and SUZ12 knockout (KO) HeLa cells, using SUZ12 antibody (A7786) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunofluorescence analysis of C6 cells using SUZ12 Polyclonal Antibody (A7786) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg SUZ12 antibody (A7786). Western blot was performed from the immunoprecipitate using SUZ12 antibody (A7786) at a dilution of 1:500.
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Key applications Western blotting, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 470-739 of human SUZ12 (NP_056170.2). KO Validated Validated Isotype IgG