![[KO Validated] YAP1 Rabbit pAb (A11264)](/upload/resize_cache/iblock/890/340_340_140cd750bba9870f18aada2478b24840a/mz6uehk5swrpnxrtjsd27ins1uiopxkl.jpg "[KO Validated] YAP1 Rabbit pAb (A11264)")
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Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] YAP1 Rabbit pAb (A11264)
- Описание
- Характеристики
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Overview
Product name [KO Validated] YAP1 Rabbit pAb Catalog No. A11264 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1). Sequence PTAQHLRQSSFEIPDDVPLPAGWEMAKTSSGQRYFLNHIDQTTTWQDPRKAMLSQMNVTAPTSPPVQQNMMNSASGPLPDGWEQAMTQDGEIYYINHKNKTTSWLDPRLDPRFAMNQRISQSAPVKQPPPLAPQSPQGGVMGGSNSNQQQQMRLQQLQMEKERLRLKQQELLRQAMRNINPSTANSPKCQELALRSQLPTLEQDGGTQNPVSSPGMSQELRTMTTNSSDPFLNSGTYHSRDESTDSGLSMSSYSVPRTPDDFLNSVDEMDTGDTINQSTLPSQQNRFPDYLEAIPGTNVDLGTLEGDGMNIEGEELMPSLQEALSSDILNDMESVLAATKLDKESFLTWL Gene ID Swiss Prot Synonyms YAP; YKI; COB1; YAP2; YAP-1; YAP65 Calculated MW 54kDa Observed MW 75kDa Applications
Reactivity Human, Mouse Tested applications WB IP Recommended dilution - WB 1:500 - 1:2000
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunoprecipitation Positive samples A-549, HeLa, BT-474, Mouse liver Cellular location Cytoplasm, Nucleus Customer validation WB(Mus musculus)
IF(Mus musculus)
Western blot analysis of extracts of various cell lines, using YAP1 antibody (A11264) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of extracts from normal (control) and YAP1 Rabbit pAb knockout (KO) HeLa cells, using YAP1 Rabbit pAb antibody (A11264) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg YAP1 antibody (A11264). Western blot was performed from the immunoprecipitate using YAP1 antibody (A11264) at a dilution of 1:1000.
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Key applications Western blotting, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1). KO Validated Validated Isotype IgG