![[KO Validated] RhoGDI Rabbit pAb (A1214)](/upload/resize_cache/iblock/cca/340_340_140cd750bba9870f18aada2478b24840a/32h2ncy3aqeuflqpou2sglcy4mq0086t.jpg "[KO Validated] RhoGDI Rabbit pAb (A1214)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] RhoGDI Rabbit pAb (A1214)
- Описание
- Характеристики
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Overview
Product name [KO Validated] RhoGDI Rabbit pAb Catalog No. A1214 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-204 of human RhoGDI (NP_001172006.1). Sequence MAEQEPTAEQLAQIAAENEEDEHSVNYKPPAQKSIQEIQELDKDDESLRKYKEALLGRVAVSADPNVPNVVVTGLTLVCSSAPGPLELDLTGDLESFKKQSFVLKEGVEYRIKISFRVNREIVSGMKYIQHTYRKGVKIDKTDYMVGSYGPRAEEYEFLTPVEEAPKGMLARGSYSIKSRFTDDDKTDHLSWEWNLTIKKDWKD Gene ID Swiss Prot Synonyms GDIA1; NPHS8; RHOGDI; RHOGDI-1; HEL-S-47e Calculated MW 23kDa Observed MW 25kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples 293T, HeLa, COS7, PC-12, Mouse lung, Mouse spleen, Mouse kidney Cellular location Cytoplasm Customer validation WB(Homo sapiens, Mus musculus, Rattus norvegicus)
Co-IP(Rattus norvegicus)
Western blot analysis of extracts from normal (control) and RhoGDI knockout (KO) 293T cells, using RhoGDI antibody (A1214) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunofluorescence analysis of HeLa cells using RhoGDI antibody (A1214). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-204 of human RhoGDI (NP_001172006.1). KO Validated Validated Isotype IgG