![[KO Validated] SHMT2 Rabbit pAb (A1215)](/upload/resize_cache/iblock/22f/340_340_140cd750bba9870f18aada2478b24840a/6xhttpuub8nlm0ooyafqbomaw0uip7pv.jpg "[KO Validated] SHMT2 Rabbit pAb (A1215)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] SHMT2 Rabbit pAb (A1215)
- Описание
- Характеристики
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Overview
Product name [KO Validated] SHMT2 Rabbit pAb Catalog No. A1215 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5, 10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 265-504 of human SHMT2 (NP_005403.2). Sequence PSPFKHADIVTTTTHKTLRGARSGLIFYRKGVKAVDPKTGREIPYTFEDRINFAVFPSLQGGPHNHAIAAVAVALKQACTPMFREYSLQVLKNARAMADALLERGYSLVSGGTDNHLVLVDLRPKGLDGARAERVLELVSITANKNTCPGDRSAITPGGLRLGAPALTSRQFREDDFRRVVDFIDEGVNIGLEVKSKTAKLQDFKSFLLKDSETSQRLANLRQRVEQFARAFPMPGFDEH Gene ID Swiss Prot Synonyms GLYA; SHMT; mSHMT; NEDCASB; HEL-S-51e Calculated MW 56kDa Observed MW 56kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, 293T, SW620 Cellular location Mitochondrion, Mitochondrion inner membrane, Mitochondrion matrix, mitochondrion nucleoid Customer validation WB(A172, Homo sapiens)
WB，IHC(Homo sapiens)
Western blot analysis of extracts of various cell lines, using SHMT2 Rabbit pAb (A1215) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Western blot analysis of extracts from normal (control) and SHMT2 Rabbit pAb knockout (KO) HeLa cells, using SHMT2 Rabbit pAb antibody (A1215) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
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Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 265-504 of human SHMT2 (NP_005403.2). KO Validated Validated Isotype IgG