![[KO Validated] SELENBP1 Rabbit pAb (A1222)](/upload/resize_cache/iblock/00e/340_340_140cd750bba9870f18aada2478b24840a/qu5uzhrqejopnlrwa56uhs1btubcoqlb.jpg "[KO Validated] SELENBP1 Rabbit pAb (A1222)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] SELENBP1 Rabbit pAb (A1222)
- Описание
- Характеристики
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Overview
Product name [KO Validated] SELENBP1 Rabbit pAb Catalog No. A1222 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a member of the selenium-binding protein family. Selenium is an essential nutrient that exhibits potent anticarcinogenic properties, and deficiency of selenium may cause certain neurologic diseases. The effects of selenium in preventing cancer and neurologic diseases may be mediated by selenium-binding proteins, and decreased expression of this gene may be associated with several types of cancer. The encoded protein may play a selenium-dependent role in ubiquitination/deubiquitination-mediated protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 243-472 of human SELENBP1 (NP_003935.2). Sequence SLKDGLIPLEIRFLHNPDAAQGFVGCALSSTIQRFYKNEGGTWSVEKVIQVPPKKVKGWLLPEMPGLITDILLSLDDRFLYFSNWLHGDLRQYDISDPQRPRLTGQLFLGGSIVKGGPVQVLEDEELKSQPEPLVVKGKRVAGGPQMIQLSLDGKRLYITTSLYSAWDKQFYPDLIREGSVMLQVDVDTVKGGLKLNPNFLVDFGKEPLGPALAHELRYPGGDCSSDIWI Gene ID Swiss Prot Synonyms MTO; LPSB; SP56; hSBP; EHMTO; SBP56; HEL-S-134P Calculated MW 52kDa Observed MW 55kDa Applications
Reactivity Human, Mouse Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples MCF7, Mouse liver, Mouse kideny Cellular location Cytoplasm, Membrane, Nucleus, Peripheral membrane protein, cytosol Customer validation IHC(Human cells)
Western blot analysis of extracts of various cell lines, using SELENBP1 antibody (A1222) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Western blot analysis of extracts from normal (control) and SELENBP1 knockout (KO) HeLa cells, using SELENBP1 antibody (A1222) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Immunofluorescence analysis of HeLa cells using SELENBP1 antibody (A1222). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 243-472 of human SELENBP1 (NP_003935.2). KO Validated Validated Isotype IgG