![[KO Validated] PHD2/EGLN1 Rabbit pAb (A14557)](/upload/resize_cache/iblock/ba1/340_340_140cd750bba9870f18aada2478b24840a/vbn4466soy5cjda8j5pkr96e0myzy041.jpg "[KO Validated] PHD2/EGLN1 Rabbit pAb (A14557)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] PHD2/EGLN1 Rabbit pAb (A14557)
- Описание
- Характеристики
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Overview
Product name [KO Validated] PHD2/EGLN1 Rabbit pAb Catalog No. A14557 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3).Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PHD2/EGLN1 (NP_071334.1). Sequence MANDSGGPGGPSPSERDRQYCELCGKMENLLRCSRCRSSFYCCKEHQRQDWKKHKLVCQGSEGALGHGVGPHQHSGPAPPAAVPPPRAGAREPRKAAARR Gene ID Swiss Prot Synonyms HPH2; PHD2; SM20; ECYT3; HALAH; HPH-2; HIFPH2; ZMYND6; C1orf12; HIF-PH2 Calculated MW 46kDa Observed MW 50kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples 293T, A-549, Mouse heart Cellular location Cytoplasm, Nucleus Customer validation WB(Mus musculus)
IF(Mus musculus)
COIP(Mus musculus)
Western blot analysis of extracts from normal (control) and PHD2/EGLN1 knockout (KO) 293T cells, using PHD2/EGLN1 antibody (A14557) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using PHD2/EGLN1 antibody (A14557) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded mouse brain using [KO Validated] PHD2/EGLN1 Rabbit pAb (A14557) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat brain using [KO Validated] PHD2/EGLN1 Rabbit pAb (A14557) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat heart using [KO Validated] PHD2/EGLN1 Rabbit pAb (A14557) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] PHD2/EGLN1 Rabbit pAb (A14557) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PHD2/EGLN1 (NP_071334.1). KO Validated Validated Isotype IgG