быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
ACTR1B Rabbit mAb (A21140)
- Описание
- Характеристики
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Overview
Product name ACTR1B Rabbit mAb Catalog No. A21140 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC3033 Background
This gene encodes a 42.3 kD subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like ACTR1A, is an actin-related protein. These two proteins, which are of equal length and share 90% amino acid identity, are present in a constant ratio of approximately 1:15 in the dynactin complex.Immunogen information
Immunogen Recombinant protein of Human ACTR1B. Sequence Email for sequence Gene ID Swiss Prot Synonyms PC3; ARP1B; CTRN2 Calculated MW 42kDa Observed MW 42kDa Applications
Reactivity Human, Rat Tested applications WB IHC-P IP Recommended dilution - WB 1:1000 - 1:5000
- IHC-P 1:50 - 1:200
- IP 1:1000 - 1:5000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunoprecipitation Positive samples HeLa, Hep G2, Jurkat, U-87MG, Rat brain Cellular location Cytoplasm, Centrosome, Cytoskeleton, Microtubule organizing center 
Western blot analysis of extracts of various cell lines, using ACTR1B antibody (A21140) at1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of extracts of Rat brain, using ACTR1B antibody (A21140) at1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded rat heart using ACTR1B Rabbit mAb (A21140) at dilution of 1:100(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat testis using ACTR1B Rabbit mAb (A21140) at dilution of 1:100(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg ACTR1B antibody (A21140). Western blot was performed from the immunoprecipitate using ACTR1B antibody (A21140) at a dilution of1:2000.
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Key applications Western blotting, Immunohistochemistry, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Крыса Immunogen Recombinant protein of Human ACTR1B. Isotype IgG