быстрая доставка ~4 недели
Размер
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ABflo® 488 Rabbit anti-Human/Mouse CD276/B7-H3 mAb (A22156)
- Описание
- Характеристики
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Overview
Product name ABflo® 488 Rabbit anti-Human/Mouse CD276/B7-H3 mAb Catalog No. A22156 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC54779-ABf488 Background
The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 29-245 of human CD276/B7-H3 (NP_079516.1). Sequence LEVQVPEDPVVALVGTDATLCCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFAEGQDQGSAYANRTALFPDLLAQGNASLRLQRVRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCSSYRGYPEAEVFWQDGQGVPLTGNVTTSQMANEQGLFDVHSVLRVVLGANGTYSCLVRNPVLQQDAHGSVTITGQPMTFP Gene ID Swiss Prot Synonyms B7H3; B7-H3; B7RP-2; 4Ig-B7-H3 Calculated MW 33kDa/52kDa/57kDa Observed MW Applications
Reactivity Human, Mouse Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location External side of plasma membrane 
Flow cytometry:1X10^6 Jurkat cells (negative control, left) and PC-3 cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human/Mouse CD276/B7-H3 mAb(A22156, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 C2C12 cells were surface-stained with ABflo® 488 Rabbit anti-Human/Mouse CD276/B7-H3 mAb(A22156, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained C2C12 cells was used as blank control (red line).
Flow cytometry:1X10^6 C2C12 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human/Mouse CD276/B7-H3 mAb(A22156, 5 μl/Test, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 29-245 of human CD276/B7-H3 (NP_079516.1). Isotype IgG