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Размер
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ABflo® 488 Rabbit anti-Human IgG (Fc) mAb (A22504)
- Описание
- Характеристики
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Overview
Product name ABflo® 488 Rabbit anti-Human IgG (Fc) mAb Catalog No. A22504 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC53383-ABf488 Background
Predicted to enable antigen binding activity and immunoglobulin receptor binding activity. Predicted to be involved in several processes, including activation of immune response; defense response to other organism; and phagocytosis. Predicted to act upstream of or within several processes, including immunoglobulin mediated immune response; positive regulation of hypersensitivity; and positive regulation of phagocytosis. Located in extracellular space.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 100-330 of human Human IgG1 (Fc) (P01857). Sequence PKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK Gene ID Swiss Prot Synonyms Calculated MW 36kDa Observed MW Refer to figures Applications
Reactivity Human Tested applications FC FC(Intra) Recommended dilution - FC(Intra) 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Positive samples Cellular location Cell membrane, Single-pass membrane protein, Secreted 
Flow cytometry:1X10^6 293F cells (negative control, left) and 293F(Transfection, right) cells were intracellularly-stained with ABflo® 488 Rabbit anti-Human IgG (Fc) mAb(A22504, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 293F(Transfection) cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human IgG (Fc) mAb(A22504, 5 μl/Test, right).
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Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 100-330 of human Human IgG1 (Fc) (P01857). Isotype IgG