быстрая доставка ~4 недели
Размер
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ABflo® 488 Rabbit anti-Human CD58 mAb (A22512)
- Описание
- Характеристики
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Overview
Product name ABflo® 488 Rabbit anti-Human CD58 mAb Catalog No. A22512 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC54829-ABf488 Background
This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 29-215 of human CD58 (NP_001770.1). Sequence FSQQIYGVVYGNVTFHVPSNVPLKEVLWKKQKDKVAELENSEFRAFSSFKNRVYLDTVSGSLTIYNLTSSDEDEYEMESPNITDTMKFFLYVLESLPSPTLTCALTNGSIEVQCMIPEHYNSHRGLIMYSWDCPMEQCKRNSTSIYFKMENDLPQKIQCTLSNPLFNTTSSIILTTCIPSSGHSRHR Gene ID Swiss Prot Synonyms ag3; LFA3; LFA-3 Calculated MW 28kDa Observed MW Refer to figures Applications
Reactivity Human Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location Cell membrane, Single-pass type I membrane protein 
Flow cytometry:1X10^6 A204 cells (Low Expression, left) and MCF7 cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human CD58 mAb(A22512, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 MCF7 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD58 mAb(A22512, 5 μl/Test, right).
Flow cytometry:1X10^6 A204 cells(Low Expression) were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD58 mAb(A22512, 5 μl/Test, right).
Flow cytometry:1X10^6 Human PBMC were surface-stained with ABflo® 488 Rabbit anti-Human CD58 mAb(A22512, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained Human PBMC was used as blank control (red line).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 29-215 of human CD58 (NP_001770.1). Isotype IgG