быстрая доставка ~4 недели
Размер
- 100 T
- 200 T
- 500 T
ABflo® 488 Rabbit anti-Human TROP-2 mAb (A22577)
- Описание
- Характеристики
-
Overview
Product name ABflo® 488 Rabbit anti-Human TROP-2 mAb Catalog No. A22577 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC51505-ABf488 Background
This intronless gene encodes a carcinoma-associated antigen. This antigen is a cell surface receptor that transduces calcium signals. Mutations of this gene have been associated with gelatinous drop-like corneal dystrophy.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 27-274 of human TROP-2 (NP_002344.2). Sequence HTAAQDNCTCPTNKMTVCSPDGPGGRCQCRALGSGMAVDCSTLTSKCLLLKARMSAPKNARTLVRPSEHALVDNDGLYDPDCDPEGRFKARQCNQTSVCWCVNSVGVRRTDKGDLSLRCDELVRTHHILIDLRHRPTAGAFNHSDLDAELRRLFRERYRLHPKFVAAVHYEQPTIQIELRQNTSQKAAGDVDIGDAAYYFERDIKGESLFQGRGGLDLRVRGEPLQVERTLIYYLDEIPPKFSMKRLT Gene ID Swiss Prot Synonyms EGP1; GP50; M1S1; EGP-1; TROP2; GA7331; GA733-1 Calculated MW 36kDa Observed MW Refer to figures Applications
Reactivity Human Tested applications IF/ICC FC Recommended dilution - IF/ICC 1:50 - 1:200
- FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Immunofluorescence Flow Cytometry Positive samples Cellular location Membrane, Single-pass type I membrane protein 
Immunofluorescence analysis of MCF7 using ABflo® 488 Rabbit anti-Human TROP-2 mAb (A22577) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
Flow cytometry:1X10^6 U-118MG cells (negative control, left) and MCF7 cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human TROP-2 mAb(A22577, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
-
Key applications Immunofluorescence, Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 27-274 of human TROP-2 (NP_002344.2). Isotype IgG