![[KD Validated] COPB2 Rabbit PolymAb® (A22646)](/upload/resize_cache/iblock/73b/340_340_140cd750bba9870f18aada2478b24840a/vl22p3yls919o6idx64yk4gciy2lf92p.jpg "[KD Validated] COPB2 Rabbit PolymAb® (A22646)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KD Validated] COPB2 Rabbit PolymAb® (A22646)
- Описание
- Характеристики
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Overview
Product name [KD Validated] COPB2 Rabbit PolymAb® Catalog No. A22646 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC52625_ARC52626 Background
The Golgi coatomer complex (see MIM 601924) constitutes the coat of nonclathrin-coated vesicles and is essential for Golgi budding and vesicular trafficking. It consists of 7 protein subunits, including COPB2.Immunogen information
Immunogen Recombinant protein of human COPB2. Sequence Email for sequence Gene ID Swiss Prot Synonyms OPDD; MCPH19; beta'-COP Calculated MW 102kDa Observed MW 102kDa Applications
Reactivity Human, Mouse Tested applications WB IF/ICC Recommended dilution - WB 1:2000 - 1:20000
- IF/ICC 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples Jurkat, MCF7, HeLa, mouse testis Cellular location COPI-coated vesicle membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Golgi apparatus membrane, Peripheral membrane protein, cytosol 
Western blot analysis of various lysates, using [KD Validated] COPB2 Rabbit PolymAb® (A22646) at 1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts from wild type(WT) and COPB2 knockdown (KD) Hela cells, using [KD Validated] COPB2 Rabbit PolymAb® (A22646) at 1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunofluorescence analysis of HeLa using [KD Validated] COPB2 Rabbit PolymAb® (A22646) at dilution of 1:500 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant protein of human COPB2. Isotype IgG