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ABflo® 488 Rabbit anti-Human/Mouse Integrin alpha 5 (ITGA5/CD49e) mAb (A22771)
- Описание
- Характеристики
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Overview
Product name ABflo® 488 Rabbit anti-Human/Mouse Integrin alpha 5 (ITGA5/CD49e) mAb Catalog No. A22771 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC58294-ABf488 Background
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.Immunogen information
Immunogen Recombinant protein of human/mouse alpha 5 (ITGA5/CD49e). Sequence FNLDAEAPAV LSGPPGSFFG FSVEFYRPGT DGVSVLVGAP KANTSQPGVL QGGAVYLCPW GASPTQCTPI EFDSKGSRLL ESSLSSSEGE EPVEYKSLQW FGATVRAHGS SILACAPLYS WRTEKEPLSD PVGTCYLSTD NFTRILEYAP CRSDFSWAAG QGYCQGGFSA EFTKTGRVVL GGPGSYFWQG QILSATQEQI AESYYPEYLI NLVQGQLQTR QASSIYDDSY LGYSVAVGEF SGDDTEDFVA GVPKGNLTYG YVTILNGSDI RSLYNFSGEQ MASYFGYAVA ATDVNGDGLD DLLVGAPLLM DRTPDGRPQE VGRVYVYLQH PAGIEPTPTL TLTGHDEFGR FGSSLTPLGD LDQDGYNDVA IGAPFGGETQ QGVVFVFPGG PGGLGSKPSQ VLQPLWAASH TPDFFGSALR GGRDLDGNGY PDLIVGSFGV DKAVVYRGRP IVSASASLTI FPAMFNPEER SCSLEGNPVA CINLSFCLNA SGKHVADSIG FTVELQLDWQ KQKGGVRRAL FLASRQATLT QTLLIQNGAR EDCREMKIYL RNESEFRDKL SPIHIALNFS LDPQAPVDSH GLRPALHYQS KSRIEDKAQI LLDCGEDNIC VPDLQLEVFG EQNHVYLGDK NALNLTFHAQ NVGEGGAYEA ELRVTAPPEA EYSGLVRHPG NFSSLSCDYF AVNQSRLLVC DLGNPMKAGA SLWGGLRFTV PHLRDTKKTI QFDFQILSKN LNNSQSDVVS FRLSVEAQAQ VTLNGVSKPE AVLFPVSDWH PRDQPQKEED LGPAVHHVYE LINQGPSSIS QGVLELSCPQ ALEGQQLLYV TRVTGLNCTT NHPINPKGLE LDPEGSLHHQ QKREAPSRSS ASSGPQILKC PEAECFRLRC ELGPLHQQES QSLQLHFRVW AKTFLQREHQ PFSLQCEAVY KALKMPYRIL PRQLPQKERQ VATAVQWTKA EGSY Gene ID Swiss Prot Synonyms FNRA; CD49e; VLA-5; VLA5A Calculated MW 115kDa Observed MW Applications
Reactivity Human, Mouse Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location Cell junction, Plasma membrane 
Flow cytometry:1X10^6 SH-SY5Y cells (negative control, Left) and U-87MG cells (Right) were surface-stained with ABflo® 488 Rabbit anti-Human/Mouse Integrin alpha 5 (ITGA5/CD49e) mAb (A22771, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 NIH/3T3 cells were surface-stained with ABflo® 488 Rabbit anti-Human/Mouse Integrin alpha 5 (ITGA5/CD49e) mAb(A22771, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained NIH/3T3 cells was used as blank control (red line).
Flow cytometry:1X10^6 U-87MG cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human/Mouse Integrin alpha 5 (ITGA5/CD49e) mAb(A22771, 5 μl/Test, right).
Flow cytometry:1X10^6 NIH/3T3 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human/Mouse Integrin alpha 5 (ITGA5/CD49e) mAb(A22771, 5 μl/Test, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant protein of human/mouse alpha 5 (ITGA5/CD49e). Isotype IgG