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Размер
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ABflo® 488 Rabbit anti-Mouse CD34 mAb (A23107)
- Описание
- Характеристики
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Overview
Product name ABflo® 488 Rabbit anti-Mouse CD34 mAb Catalog No. A23107 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC58336-ABf488 Background
Enables sulfate binding activity. Acts upstream of or within leukocyte migration. Located in cytoplasm; external side of plasma membrane; and extracellular region. Is expressed in several structures, including alimentary system; cardiovascular system; extraembryonic component; genitourinary system; and skin. Orthologous to human CD34 (CD34 molecule).Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 35-287 of mouse CD34(NP_598415.1) Sequence NNGTATPELPTQGTFSNVSTNVSYQETTTPSTLGSTSLHPVSQHGNEATTNITETTVKFTSTSVITSVYGNTNSSVQSQTSVISTVFTTPANVSTPETTLKPSLSPGNVSDLSTTSTSLATSPTKPYTSSSPILSDIKAEIKCSGIREVKLTQGICLEQNKTSSCAEFKKDRGEGLARVLCGEEQADADAGAQVCSLLLAQSEVRPQCLLLVLANRTEISSKLQLMKKHQSDLKKLGILDFTEQDVASHQSYS Gene ID Swiss Prot Synonyms CD34; CD34 molecule; GIG3; MORT1 Calculated MW 41kDa Observed MW Applications
Reactivity Mouse Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location Plasma membrane 
Flow cytometry:1X10^6 Neuro-2a cells (negative control, Left) and NIH/3T3 cells (Right) were surface-stained with ABflo® 488 Rabbit anti-Mouse CD34 mAb(A23107, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells was used as blank control (red line).
Flow cytometry:1X10^6 NIH/3T3 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse CD34 mAb(A23107, 5 μl/Test, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 35-287 of mouse CD34(NP_598415.1) Isotype IgG