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Размер
- 100 T
- 200 T
- 500 T
ABflo® 488 Rabbit anti-Human IgD mAb (A23112)
- Описание
- Характеристики
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Overview
Product name ABflo® 488 Rabbit anti-Human IgD mAb Catalog No. A23112 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC60051-ABf488 Background
IgD is the major antigen receptor isotype on the surface of most peripheral B-cells, where it is coexpressed with IgM. The membrane-bound IgD (mIgD) induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 99-384 of human IgD Sequence RWPESPKAQASSVPTAQPQAEGSLAKATTAPATTRNTGRGGEEKKKEKEKEEQEERETKTPECPSHTQPLGVYLLTPAVQDLWLRDKATFTCFVVGSDLKDAHLTWEVAGKVPTGGVEEGLLERHSNGSQSQHSRLTLPRSLWNAGTSVTCTLNHPSLPPQRLMALREPAAQAPVKLSLNLLASSDPPEAASWLLCEVSGFSPPNILLMWLEDQREVNTSGFAPARPPPQPRSTTFWAWSVLRVPAPPSPQPATYTCVVSHEDSRTLLNASRSLEVSYVTDHGPMK Gene ID Swiss Prot Synonyms Calculated MW 42kDa Observed MW Applications
Reactivity Human Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location Plasma membrane, Secreted 
Flow cytometry:1X10^6 Jurkat cells (negative control, Left) and Human PBMC (Right) were surface-stained with ABflo® 488 Rabbit anti-Human IgD mAb(A23112, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells was used as blank control (red line).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 99-384 of human IgD Isotype IgG