![[KD Validated] BUB1 Rabbit mAb (A23198)](/upload/resize_cache/iblock/eb1/340_340_140cd750bba9870f18aada2478b24840a/xef4wuhnvfizydgrceknxlorfo10lwdh.jpg "[KD Validated] BUB1 Rabbit mAb (A23198)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KD Validated] BUB1 Rabbit mAb (A23198)
- Описание
- Характеристики
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Overview
Product name [KD Validated] BUB1 Rabbit mAb Catalog No. A23198 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC59688 Background
This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants.Immunogen information
Immunogen Recombinant protein of human BUB1. Sequence Email for sequence Gene ID Swiss Prot Synonyms BUB1A; BUB1L; hBUB1; MCPH30 Calculated MW 115KDa/120KDa/122KDa Observed MW 122kDa Applications
Reactivity Human, Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HEL, 293T Cellular location Chromosome, Nucleus, centromere, kinetochore 
Western blot analysis of HEL, using BUB1 Rabbit mAb (A23198) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Western blot analysis of extracts from wild type(WT) and BUB1 Rabbit mAb knockdown (KD) 293T(KD) cells, using BUB1 Rabbit mAb (A23198) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant protein of human BUB1. Isotype IgG