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Размер
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ABflo® 488 Rabbit anti-Human Claudin18.2 mAb (A23391)
- Описание
- Характеристики
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Overview
Product name ABflo® 488 Rabbit anti-Human Claudin18.2 mAb Catalog No. A23391 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC60744-ABf488 Background
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human Claudin18.2 (NP_001002026.1). Sequence MAVTACQGLGFVVSLIGIAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDEVQSYPSKHDYV Gene ID Swiss Prot Synonyms SFTA5; SFTPJ Calculated MW 27kDa Observed MW Refer to figures Applications
Reactivity Human Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location Cell junction, Cell membrane, Multi-pass membrane protein, tight junction 
Flow cytometry:1X10^6 CHO cells(negative control, left) and CHO(Transfection, right) cells were surface-stained with ABflo® 488 Rabbit anti-Human Claudin18.2 mAb(A23391, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained cells was used as blank control (red line).
Flow cytometry:1X10^6 CHO(Transfection) cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human Claudin18.2 mAb(A23391, 5 μl/Test, right).
Flow cytometry:1X10^6 NUGC4 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human Claudin18.2 mAb(A23391, 5 μl/Test, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human Claudin18.2 (NP_001002026.1). Isotype IgG