быстрая доставка ~4 недели
Размер
- 100 T
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- 500 T
ABflo® 594 Rabbit anti-Mouse CD31 mAb (A23703)
- Описание
- Характеристики
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Overview
Product name ABflo® 594 Rabbit anti-Mouse CD31 mAb Catalog No. A23703 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC61018-ABf594 Background
Predicted to enable protein homodimerization activity; protein phosphatase binding activity; and transmembrane signaling receptor activity. Acts upstream of or within several processes, including Rho protein signal transduction; endothelial cell morphogenesis; and positive regulation of tyrosine phosphorylation of STAT protein. Located in several cellular components, including cell-cell contact zone; external side of plasma membrane; and smooth muscle contractile fiber. Is expressed in several structures, including alimentary system; cardiovascular system; central nervous system; extraembryonic component; and genitourinary system. Human ortholog(s) of this gene implicated in coronary artery disease (multiple); lung non-small cell carcinoma; neuroblastoma; and psoriatic arthritis. Orthologous to human PECAM1 (platelet and endothelial cell adhesion molecule 1).Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 18-220 of mouse CD31 (NP_001292086.1). Sequence EENSFTINSIHMESLPSWEVMNGQQLTLECLVDISTTSKSRSQHRVLFYKDDAMVYNVTSREHTESYVIPQARVFHSGKYKCTVMLNNKEKTTIEYEVKVHGVSKPKVTLDKKEVTEGGVVTVNCSLQEEKPPIFFKIEKLEVGTKFVKRRIDKTSNENFVLMEFPIEAQDHVLVFRCQAGILSGFKLQESEPIRSEYVTVQE Gene ID Swiss Prot Synonyms Cd31; Pecam; PECAM-1 Calculated MW 69kDa/77kDa/80kDa/81kDa Observed MW Applications
Reactivity MOUSE Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location Cell surface, cytoplasm, external side of plasma membrane, extracellular space, plasma membrane 
Flow cytometry:1X10^6 NIH/3T3 cells(negative control, left) and C57BL/6 mouse Splenocytes(right) were surface-stained with ABflo® 594 Rabbit anti-Mouse CD31 mAb(A23703, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 C57BL/6 mouse Splenocytes were surface-stained with ABflo® 594 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 594 Rabbit anti-Mouse CD31 mAb(A23703, 5 μl/Test, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 18-220 of mouse CD31 (NP_001292086.1). Isotype IgG