быстрая доставка ~4 недели
Размер
- 100 T
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ABflo® 594 Rabbit anti-Human CD80/B7-1 mAb (A23731)
- Описание
- Характеристики
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Overview
Product name ABflo® 594 Rabbit anti-Human CD80/B7-1 mAb Catalog No. A23731 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC61136-ABf594 Background
The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 35-242 of human CD80/B7-1 (NP_005182.1). Sequence VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDN Gene ID Swiss Prot Synonyms CD80; B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; LAB7; CD80 molecule Calculated MW 18kDa/29kDa/33kDa Observed MW Applications
Reactivity Human Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location Cell surface, external side of plasma membrane, plasma membrane 
Flow cytometry: 1X10^6 293F cells (negative control, left) and Raji cells (right) were surface-stained with ABflo® 594 Rabbit anti-Human CD80/B7-1 mAb(A23731, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 Raji cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 594 Rabbit anti-Human CD80/B7-1 mAb(A23731, 5 μl/Test, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 35-242 of human CD80/B7-1 (NP_005182.1). Isotype IgG