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ABflo® 488 Rabbit anti-Human/Mouse CD55/DAF mAb (A23897)
- Описание
- Характеристики
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Overview
Product name ABflo® 488 Rabbit anti-Human/Mouse CD55/DAF mAb Catalog No. A23897 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC60934-ABflo488 Background
This gene encodes an inhibitor of both the classical and the alternative pathways of complement activation. The encoded preproprotein undergoes post-translational processing to generate a mature polypeptide anchored to the plasma membrane via a glycosylphosphatidylinositol moiety. Erythrocytes from mice deficient in the encoded protein exhibit impaired regulation of complement activation resulting in enhanced complement deposition. Mice lacking the encoded protein exhibit enhanced susceptibility to experimentally induced myasthenia gravis. This gene is located adjacent to a closely related gene on chromosome 1.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 35-361 of mouse CD55/DAF（NP_034146.2）. Sequence DCGPPPDIPNARPILGRHSKFAEQSKVAYSCNNGFKQVPDKSNIVVCLENGQWSSHETFCEKSCVAPERLSFASLKKEYLNMNFFPVGTIVEYECRPGFRKQPPLPGKATCLEDLVWSPVAQFCKKKSCPNPKDLDNGHINIPTGILFGSEINFSCNPGYRLVGVSSTFCSVTGNTVDWDDEFPVCTEIHCPEPPKINNGIMRGESDSYTYSQVVTYSCDKGFILVGNASIYCTVSKSDVGQWSSPPPRCIEKSKVPTKKPTINVPSTGTPSTPQKPTTESVPNPGDQPTPQKPSTVKVSATQHVPVTKTTVRHPIRTSTDKGEPNT Gene ID Swiss Prot Synonyms Daf; Daf1; Daf-GPI; GPI-DAF Calculated MW 42KDa Observed MW Applications
Reactivity Human, Mouse Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location Cell membrane 
Flow cytometry: 1X10^6 C57BL/6 mouse Splenocytes were surface-stained with ABflo® 488 Rabbit anti-Human/Mouse CD55/DAF mAb(A23897, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained C57BL/6 mouse Splenocytes were used as blank control (red line).
Flow cytometry: 1X10^6 SH-SH5Y cells(negative control, left) and Hela cells(right) were surface-stained with ABflo® 488 Rabbit anti-Human/Mouse CD55/DAF mAb(A23897, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line). ； Flow cytometry: 1X10^6 C57BL/6 mouse Splenocytes were surface-stained with ABflo® 488 Rabbit anti-Human/Mouse CD55/DAF mAb(A23897, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained C57BL/6 mouse Splenocytes were used as blank control (red line).
Flow cytometry:1X10^6 C57BL/6 mouse Splenocytes were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human/Mouse CD55/DAF mAb(A23897, 5 μl/Test, right).
Flow cytometry:1X10^6 Hela cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human/Mouse CD55/DAF mAb(A23897, 5 μl/Test, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 35-361 of mouse CD55/DAF（NP_034146.2）. Isotype IgG