быстрая доставка ~4 недели
Размер
- 100 T
- 200 T
- 500 T
ABflo® 488 Rabbit anti-Mouse CD80 mAb (A23965)
- Описание
- Характеристики
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Overview
Product name ABflo® 488 Rabbit anti-Mouse CD80 mAb Catalog No. A23965 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC61485-ABflo488 Background
Predicted to enable coreceptor activity. Acts upstream of or within T cell costimulation; cellular response to lipopolysaccharide; and positive regulation of alpha-beta T cell proliferation. Located in external side of plasma membrane. Is expressed in heart ventricle and skin. Human ortholog(s) of this gene implicated in type 1 diabetes mellitus. Orthologous to human CD80 (CD80 molecule).Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to aminoacids 38-245 of mouse CD80(NP_033985.3). Sequence VDEQLSKSVKDKVLLPCRYNSPHEDESEDRIYWQKHDKVVLSVIAGKLKVWPEYKNRTLYDNTTYSLIILGLVLSDRGTYSCVVQKKERGTYEVKHLALVKLSIKADFSTPNITESGNPSADTKRITCFASGGFPKPRFSWLENGRELPGINTTISQDPESELYTISSQLDFNTTRNHTIKCLIKYGDAHVSEDFTWEKPPEDPPDSK Gene ID Swiss Prot Synonyms B71; Ly53; TSA1; Cd28l; Ly-53; MIC17 Calculated MW 34kDa Observed MW Applications
Reactivity Mouse Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location Membrane, Single-pass type I membrane protein 
Flow cytometry:1X10^6 Neuro-2a cells (negative control, left) and C2C12 cells (right) were surface-stained with ABflo® 488 Rabbit anti-Mouse CD80 mAb(A23965, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 C2C12 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse CD80 mAb(A23965, 5 μl/Test, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to aminoacids 38-245 of mouse CD80(NP_033985.3). Isotype IgG