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ABflo® 488 Rabbit anti-Human β2 Microglobulin mAb (A24223)
- Описание
- Характеристики
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Overview
Product name ABflo® 488 Rabbit anti-Human β2 Microglobulin mAb Catalog No. A24223 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC60950-ABflo488 Background
This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human β2 Microglobulin(NP_004039.1). Sequence IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM Gene ID Swiss Prot Synonyms B2M; IMD43; beta-2-microglobulin Calculated MW 13kDa Observed MW Refer to figures Applications
Reactivity Human Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location Secreted, Cell surface 
Flow cytometry: 1X10^6 Daudi cells (negative control, left) and HeLa (right) were surface-stained with ABflo® 488 Rabbit anti-Human β2 Microglobulin mAb(A24223, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained Daudi cells were used as blank control (red line).
Flow cytometry:1X10^6 HeLa were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human β2 Microglobulin mAb(A24223, 5 μl/Test, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human β2 Microglobulin(NP_004039.1). Isotype IgG