быстрая доставка ~4 недели
Размер
- 100 T
- 200 T
- 500 T
ABflo® 594 Rabbit anti-FITC/5-FAM/6-FAM mAb (A24240)
- Описание
- Характеристики
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Overview
Product name ABflo® 594 Rabbit anti-FITC/5-FAM/6-FAM mAb Catalog No. A24240 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC51071-04-ABf647 Background
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.Immunogen information
Immunogen Chemical/ Small Molecule corresponding to FITC/5-FAM/6-FAM. Sequence Email for sequence Gene ID Swiss Prot Synonyms FITC Calculated MW Observed MW Applications
Reactivity Species independent Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location 
Flow cytometry:1X10^6 HT-29 cells (negative control, left) and Raji cells (right) were surface-stained with Mouse anti-Human CD86 mAb(Biolegend 374203, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line), then stained with ABflo® 594 Rabbit anti-FITC/5-FAM/6-FAM mAb(A24240, 5 μl/Test）, Non-fluorescently stained cells were used as blank control (red line).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Независимый от вида Immunogen Chemical/ Small Molecule corresponding to FITC/5-FAM/6-FAM. Isotype IgG