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ABflo® 488 Rabbit anti-Human Integrin β5/ITGB5 mAb (A24246)
- Описание
- Характеристики
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Overview
Product name ABflo® 488 Rabbit anti-Human Integrin β5/ITGB5 mAb Catalog No. A24246 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC60858-ABflo488 Background
Ιntegrins, heterodimeric trans-membrane matrix receptors, are mainly involved in the signal transduction and attachments to extra cellular matrix (ECM).†† In ECM they act as a mediator of cell adhesion.† In human, at least 18 α and eight β subunits have been found.† ITGB5 (integrin subunit β 5) helps in facilitation of cancer cell migration, anchorage-independent growth and tumor angiogenesis. Integrin is activated by G-protein-coupled receptors, resulting in the phosphorylation of cytoplasmic domain of the β subunit. The coupled α and β cytoplasmic tails are responsible for maintaining integrin in inactive state.Immunogen information
Immunogen Recombinant Protein corresponding to a sequence within amino acids 24-490 of human Integrin β5/ITGB5 (NP_002204.2). Sequence GLNICTSGSATSCEECLLIHPKCAWCSKEDFGSPRSITSRCDLRANLVKNGCGGEIESPASSFHVLRSLPLSSKGSGSAGWDVIQMTPQEIAVNLRPGDKTTFQLQVRQVEDYPVDLYYLMDLSLSMKDDLDNIRSLGTKLAEEMRKLTSNFRLGFGSFVDKDISPFSYTAPRYQTNPCIGYKLFPNCVPSFGFRHLLPLTDRVDSFNEEVRKQRVSRNRDAPEGGFDAVLQAAVCKEKIGWRKDALHLLVFTTDDVPHIALDGKLGGLVQPHDGQCHLNEANEYTASNQMDYPSLALLGEKLAENNINLIFAVTKNHYMLYKNFTALIPGTTVEILDGDSKNIIQLIINAYNSIRSKVELSVWDQPEDLNLFFTATCQDGVSYPGQRKCEGLKIGDTASFEVSLEARSCPSRHTEHVFALRPVGFRDSLEVGVTYNCTCGCSVGLEPNSARCNGSGTYVCGLCECS Gene ID Swiss Prot Synonyms ITGB5; integrin beta-5 Calculated MW 88kDa Observed MW Refer to figures Applications
Reactivity Human Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location Membrane 
Flow cytometry: 1X10^6 Jurkat cells(negative control, left) and A549 cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human Integrin β5/ITGB5 mAb(A24246, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained were used as blank control (red line).
Flow cytometry:1X10^6 A549 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human Integrin β5/ITGB5 mAb(A24246, 5 μl/Test, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant Protein corresponding to a sequence within amino acids 24-490 of human Integrin β5/ITGB5 (NP_002204.2). Isotype IgG