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Размер
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ABflo® 488 Rabbit anti-Human CD62E/E-Selectin mAb (A24308)
- Описание
- Характеристики
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Overview
Product name ABflo® 488 Rabbit anti-Human CD62E/E-Selectin mAb Catalog No. A24308 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC61987-ABflo488 Background
The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 22-230 of human CD62E/E-Selectin (NP_000441.2). Sequence WSYNTSTEAMTYDEASAYCQQRYTHLVAIQNKEEIEYLNSILSYSPSYYWIGIRKVNNVWVWVGTQKPLTEEAKNWAPGEPNNRQKDEDCVEIYIKREKDVGMWNDERCSKKKLALCYTAACTNTSCSGHGECVETINNYTCKCDPGFSGLKCEQIVNCTALESPEHGSLVCSHPLGNFSYNSSCSISCDRGYLPSSMETMQCMSSGEW Gene ID Swiss Prot Synonyms SELE; CD62E; ELAM; ELAM1; ESEL; LECAM2; E-selectin Calculated MW 66kDa Observed MW Refer to figures Applications
Reactivity Human Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location Cell membrane, Single-pass type I membrane protein 
Flow cytometry:1X10^6 HUVEC cells (Low Expression, Left) and HUVEC cells(treated with TNF-α, Right) were surface-stained with ABflo® 488 Rabbit anti-Human CD62E/E-Selectin mAb(A24308, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 HUVEC cells(treated with TNF-α, Right) were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD62E/E-Selectin mAb(A24308, 5 μl/Test, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 22-230 of human CD62E/E-Selectin (NP_000441.2). Isotype IgG