быстрая доставка ~4 недели
Размер
- 100 T
- 200 T
- 500 T
ABflo® 594 Rabbit anti-Mouse CD146 mAb (A24399)
- Описание
- Характеристики
-
Overview
Product name ABflo® 594 Rabbit anti-Mouse CD146 mAb Catalog No. A24399 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC62356-ABflo594 Background
Predicted to be involved in angiogenesis and positive regulation of cell migration. Predicted to act upstream of or within heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules. Predicted to be located in external side of plasma membrane; extracellular space; and nucleus. Predicted to be active in plasma membrane. Is expressed in several structures, including adipose tissue; genitourinary system; gut; nervous system; and respiratory system. Orthologous to human MCAM (melanoma cell adhesion molecule).Immunogen information
Immunogen Recombinant Protein corresponding to a sequence within amino acids 24-563 of mouse CD146 (NP_075548.2) Sequence VPGEEKQPVPTPDLVEAEVGSTALLKCGPSRASGNFSQVDWFLIHKERQILIFRVHQGKGQREPGEYEHRLSLQDSVATLALSHVTPHDERMFLCKSKRPRLQDHYVELQVFKAPEEPTIQANVVGIHVDRQELREVATCVGRNGYPIPQVLWYKNSLPLQEEENRVHIQSSQIVESSGLYTLKSVLSARLVKEDKDAQFYCELSYRLPSGNHMKESKEVTVPVFYPAEKVWVEVEPVGLLKEGDHVTIRCLTDGNPQPHFTINKKDPSTGEMEEESTDENGLLSLEPAEKHHSGLYQCQSLDLETTITLSSDPLELLVNYVSDVQVNPTAPEVQEGESLTLTCEAESNQDLEFEWLRDKTGQLLGKGPVLQLNNVRREAGGRYLCMASVPRVPGLNRTQLVSVGIFGSPWMALKERKVWVQENAVLNLSCEASGHPQPTISWNVNGSATEWNPDPQTVVSTLNVLVTPELLETGAECTASNSLGSNTTTIVLKLVTLTTLIPDSSQTTGLSTLTVSPHTRANSTSTEKKLPQPESKGVV Gene ID Swiss Prot Synonyms CD146; CD149; Muc18; s-endo; 1-gicerin; s-gicerin Calculated MW 71kDa Observed MW Refer to figures Applications
Reactivity Mouse Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location Membrane 
Flow cytometry:1X10^6 CTLL-2 cells (negative control, Left) and bEnd.3 cells (Right) were surface-stained with ABflo® 594 Rabbit anti-Mouse CD146 mAb(A24399, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 bEnd.3 cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Mouse CD146 mAb(A24399, 5 μl/Test, right).
-
Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Мышь Immunogen Recombinant Protein corresponding to a sequence within amino acids 24-563 of mouse CD146 (NP_075548.2) Isotype IgG