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ABflo® 594 Rabbit anti-Human CD166/ALCAM mAb (A24582)
- Описание
- Характеристики
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Overview
Product name ABflo® 594 Rabbit anti-Human CD166/ALCAM mAb Catalog No. A24582 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC57933-ABflo594 Background
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 28-526 of human CD166/ALCAM(NP_001618.2) Sequence WYTVNSAYGD TIIIPCRLDV PQNLMFGKWK YEKPDGSPVF IAFRSSTKKS VQYDDVPEYK DRLNLSENYT LSISNARISD EKRFVCMLVT EDNVFEAPTI VKVFKQPSKP EIVSKALFLE TEQLKKLGDC ISEDSYPDGN ITWYRNGKVL HPLEGAVVII FKKEMDPVTQ LYTMTSTLEY KTTKADIQMP FTCSVTYYGP SGQKTIHSEQ AVFDIYYPTE QVTIQVLPPK NAIKEGDNIT LKCLGNGNPP PEEFLFYLPG QPEGIRSSNT YTLTDVRRNA TGDYKCSLID KKSMIASTAI TVHYLDLSLN PSGEVTRQIG DALPVSCTIS ASRNATVVWM KDNIRLRSSP SFSSLHYQDA GNYVCETALQ EVEGLKKRES LTLIVEGKPQ IKMTKKTDPS GLSKTIICHV EGFPKPAIQW TITGSGSVIN QTEESPYING RYYSKIIISP EENVTLTCTA ENQLERTVNS LNVSAISIPE HDEADEISDE NREKVNDQA Gene ID Swiss Prot Synonyms ALCAM; CD166; MEMD; CD166 antigen Calculated MW 15kDa/33kDa/63kDa/65kDa Observed MW Applications
Reactivity Human Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location Membrane, Secreted, Single-pass type I membrane protein 
Flow cytometry:1X10^6 K-562 cells (negative control, left) and SH-SY5Y cells (right) were surface-stained with ABflo® 594 Rabbit anti-Human CD166/ALCAM mAb(A24582, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 SH-SY5Y cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human CD166/ALCAM mAb(A24582, 5 μl/Test, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 28-526 of human CD166/ALCAM(NP_001618.2) Isotype IgG