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[KD Validated] eIF4E Rabbit pAb (A22681)

быстрая доставка ~4 недели

Размер

100 μL
200 μL
50 μL

Описание
Характеристики

Overview

Product name	[KD Validated] eIF4E Rabbit pAb
Catalog No.	A22681
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant protein of human eIF4E.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1
Calculated MW	25kDa
Observed MW	29kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC
Recommended dilution	WB 1:100 - 1:500 IF/ICC 1:50 - 1:200 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunohistochemistry
Positive samples	293T, HeLa, Jurkat, RAW264.7, NIH/3T3, PC-12
Cellular location	chromatoid body, cytoplasm, cytoplasmic ribonucleoprotein granule, cytoplasmic stress granule, cytosol, eukaryotic translation initiation factor 4F complex, extracellular exosome, glutamatergic synapse, nucleus, P-body, perinuclear region of cytoplasm, postsynaptic cytosol

Western blot analysis of various lysates, using eIF4E antibody (A22681) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of extracts from wild type(WT) and eIF4E knockdown (KD) 293T(KD) cells, using eIF4E antibody (A22681) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Immunohistochemistry analysis of paraffin-embedded Rat testis using EIF4E antibody (A22681) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Mouse liver using EIF4E antibody (A22681) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Human liver using EIF4E antibody (A22681) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of HeLa cells using [KD Validated] eIF4E Rabbit pAb (A22681) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HepG2 cells using [KD Validated] eIF4E Rabbit pAb (A22681) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using [KD Validated] eIF4E Rabbit pAb (A22681) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using [KD Validated] eIF4E Rabbit pAb (A22681) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Key applications	Western blotting, Immunofluorescence, Immunohistochemistry
Host species	Кролик
Antibody type	Primary Antibodies
Reactivity	Человек, Мышь, Крыса
Immunogen	Recombinant protein of human eIF4E.
Isotype	IgG