![[KD Validated] beta 2 Microglobulin Rabbit mAb (A23430)](/upload/resize_cache/iblock/6fd/340_340_140cd750bba9870f18aada2478b24840a/k91ikodza7gk8wt7u9eam2og22jw1c5v.jpg "[KD Validated] beta 2 Microglobulin Rabbit mAb (A23430)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KD Validated] beta 2 Microglobulin Rabbit mAb (A23430)
- Описание
- Характеристики
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Overview
Product name [KD Validated] beta 2 Microglobulin Rabbit mAb Catalog No. A23430 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC60950 Background
This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human β2 Microglobulin(NP_004039.1). Sequence IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM Gene ID Swiss Prot Synonyms B2M; IMD43; beta-2-microglobulin Calculated MW 13kDa Observed MW 12kDa Applications
Reactivity Human Tested applications WB IF/ICC FC Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
- FC 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Flow Cytometry Positive samples THP-1, HeLa, U-937 Cellular location Secreted, Cell surface 
Western blot analysis of various lysates, using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts from wild type(WT) and β2-microglobulin knockdown (KD) HeLa(KD) cells, using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence analysis of HeLa using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Flow cytometry:1X10^6 Daudi cells(negative control, left) and Hela cells (right) were surface-stained with [KD Validated] beta Microglobulin Rabbit mAb(A23430, 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, blue line).Non-fluorescently stained cells was used as blank control (red line).
Flow cytometry:1X10^6 HeLa cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, left) or β2 Microglobulin Rabbit mAb(A23430, 2 μg/mL, right).
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Key applications Western blotting, Immunofluorescence, Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human β2 Microglobulin(NP_004039.1). Isotype IgG