быстрая доставка ~4 недели
Размер
- 16000 U
- 80000 U
- Описание
-
Description
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. T4 DNA Ligase is applicable to cloning of restriction fragments and to joining linkers and adapters to blunt-ended DNA.Product Components
Components Cat. No. T4 DNA Ligase RM21501 10X T4 DNA ligase Reaction Buffer RM20108 Product Information
Feature Specification Product Source An E.coli strain that carries the T4 DNA ligase gene. Applications Cloning Ligation Unit Definition One unit is defined as the amount of enzyme required to ligate 50% ligase of HindIII digestion fragments of λ DNA (5´ DNA termini concentration of 0.12 µM, 300- µg/ml) in a total reaction volume of 20 μl over 30 minutes at 16°C in 1X T4 DNA Ligase Reaction Buffer. Storage Conditions 10 mM Tris-HCl，50 mM KCl，1 mM DTT，0.1 mM EDTA，50% Glycerol，pH7.4 @ 25°C Storage Temperature -20°C Reaction Conditions 1X T4 DNA Ligase Reaction Buffer. Reaction Buffer or Mix 1X T4 DNA Ligase Reaction Buffer:
50 mM Tris-HCl，10 mM MgCl2，10 mM DTT，1 mM ATP，pH7.5 @ 25°Heat Inactivation 65°C for 10 min.