LB (Luria-Bertani) liquid medium
It shall be added into 100ml ddH2O, the components are as follows:
Tryptone 1%;
Yesat extract 0.5%;
NaCl 1%;
Adjust pH to 7.0 and constant volume to 100mL with 1M NaOH. It can be used after autoclaved at 121℃ for 20min.

NaCl 1%;
Agar powder 2%;
Adjust pH to 7.0 and constant volume to 100mL with 1M NaOH
High-pressure sterilization shall be carried out at 121℃for 20min; Inverting the plate when the temperature cooled to 50-60℃.
The temperature of the medium should not be too high, otherwise a lot of condensed water will be generated after the plate is cooled.