Recombinant RNase A

Product introduction

Recombinant RNase A is a recombinant ribonuclease that specifically attacks the 3 'end of cytosine (c) or uracil (U) residues on single stranded RNA and cuts the phosphodiester bond formed with adjacent nucleotides. Recombinant RNase A has the highest activity in cleaving single stranded RNA and is active under a variety of reaction conditions: it can be used to cleave single stranded RNA, double stranded RNA and RNA strands in RNA-DNA hybridization at low salt concentration (0-100 mM NaCl), while it can only specifically cleave single stranded RNA at high salt concentration (≥ 300 mM NaCl). This product has HIS label.

Main uses: recombinant RNase A is used to prepare DNA without host RNA; It can minimize the risk of pathogen introduction into biological process; Other molecular biology and cell biology research experiments.

Features: specific degradation of RNA, recombinant expression and purification, no animal source pollution, and strict control and test in pollution nonspecific nuclease and protease activity.

Source: RNase A gene of bovine pancreas, recombinant expression of Pichia pastoris.

Definition of enzyme activity: in 1 ml reaction system at room temperature, the amount of enzyme required to increase a286 value by 0.0146 per minute is defined as 1 enzyme activity unit. Determination conditions: 100 mm Tris acetate, 1 mm EDTA, 1 mm cyclic 2 ', 3' - CMP, pH 6.5.

Purity and concentration: SDS-PAGE detection purity ≥ 95%, no DNase, protein, 10 mg / ml.

Enzyme storage buffer: 50 mm Tris HCl, 50% glycorol, pH 7 4@25 ℃。

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Storage and transportation

Transport with wet ice; storage at -20°C, valid for 12 months.