быстрая доставка ~4 недели
Размер
- 10 μg
- 100 μg
- 20 μg
- 50 μg
- 500 μg
Active Recombinant Human IL-6RA/CD126 Protein (RP01396)
- Описание
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Overview
Product name Active Recombinant Human IL-6RA/CD126 Protein Catalog No. RP01396 Description Active Recombinant Human IL-6RA/CD126 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu20-Asp358) of human IL-6R alpha/CD126 (Accession #NP_000556.1) fused with a Fc, 6×His tag at the C-terminus. Bio-Activity Measured by its binding ability in a functional ELISA. Immobilized Human IL6 at 1 μg/mL (100 μL/well) can bind Human IL6RA with a linear range of 1-5.4 ng/mL. Purity > 95% by SDS-PAGE. Endotoxin <1EU/ug Formulation Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Species Human Background
The soluble form of recombinant human IL6R consists of 357 amino acids with a molecular weight of 40 kDa. It migrates with an apparent molecular mass of 60-65 kDa due to glycosylation in SDS-PAGE under reducing conditions. Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a potent pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and is known particularly for its role in the immune response and acute phase reactions. The low concentration of a soluble form of IL-6 receptor (sIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a sIL-6R protein are able to mediate IL-6 signals into the cells through the interaction of gp13. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp13 and inducing intracellular signalling. Through this so-called "trans-signalling" mechanism, IL-6 is able to stimulate cells that lack an endogenous mIL-6R. Dysregulated production of IL6 and IL6R are implicated in the pathogenesis of several inflammatory diseases and malignancies, and it has been reported that a humanized anti-IL6R monoclonal antibody is a promising agent applicable to the therapeutic approach for IL6 driven diseases.Product information
Synonym IL6R; CD126; IL-6R-1; IL-6RA; IL6RA; IL-6R-alpha Expression Host HEK293 cells Tag C-hFc&His Swiss-Prot Gene ID Usage guide
Storage Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.Reconstitution Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. * For research use only. Not for therapeutic or diagnostic purposes.
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